Background and objective: Hospital information system (HIS) is emerging as an essential component of healthcare. In the era of modern technology, hospitals need to be computerized to maintain electronic records of patients like admission, transfer, discharge, and death. The knowledge and attitudes of nursing personnel influence their effective use of the HIS. This study was designed to assess the knowledge and attitude among nursing personnel in using HIS. Materials and methods: Three hundred and fifty nursing personnel working in PGIMER were selected using a stratified random selection procedure using their employee numbers. Their knowledge and attitude were assessed by a validated questionnaire. Results and analysis: Out of 350 nursing personnel, 11% female and 73% male staff had used computers for more than 2 years. Thirty six percentage of males and 28% of female nursing staff got formal training from the Computer Department of PGIMER, Chandigarh. Fifty-nine percentage of males spent more than one hour daily in using computers. Less than 50% of subjects were able to answer the questions related to computers and HIS correctly. Male staff had more knowledge than females. Sixty-eight percentage of subjects had attitude that HIS does not help in reducing the duplication of work. Conclusion: The present study shows that most of the subjects were females, however, more males were using computers and spent more time on it than their counterparts spend.