Aims and background: This study was undertaken to assess the existing level of knowledge related to the prevention and identification of acute kidney injury (AKI) among nursing officers and to assess the efficacy of capacity-building intervention (CBI). An attempt was also made to find the association of participants’ profiles with knowledge scores. Materials and methods: A total of 50 nursing officers were taken from critical care units of Government Medical College and Hospital Sector-32 (GMCH-32), Chandigarh, India. Preexperimental research design was used to assess the knowledge regarding the early identification and prevention of AKI using a self-structured questionnaire. Further, participants were provided with intervention, and after 1 week, a posttest was obtained from them. Results: Statistical data revealed that the majority of the participants acquired average knowledge in their pretest, whereas only a few had good knowledge. It was observed that only 18% had good knowledge regarding the concept of AKI, 12% regarding causes, signs, and symptoms, 6% had related to identification and prevention, and 10% had related to care in their pretest. Whereas in the posttest, 38% had good knowledge related to the concept, 44% (causes, signs, and symptoms), 48% (identification and prevention), and about 28% (care). The association was found between years of experience and that of pretest knowledge. Conclusion: According to the findings of this study, the knowledge of the participants was considerably enhanced after providing CBI in the form of video. Nursing officers had improved their scores in posttest compared to pretest scores. Clinical significance: This study helps the subjects in clinical settings to identify the cases of AKI early, thus reducing the incidence.